The Analytical Services Laboratory is supported
funded primarily from the faculty researchers of SAES.
The Analytical Services Laboratory
(ASL) staff analyzed approximately 11,000 samples for over 22,000 analytes
in this reporting year with an estimated commercial value of over $380,000
surpassing last years production for dollar volume but 10% fewer
samples. The reason for this
apparent discrepancy is that more organic analyses were performed in 2001
than in 2000. The inorganic and elemental analyses decreased in 2001. The
laboratory manager is serving on seven theses committees five more
than the previous year. Six MSc. candidates worked in ASL for the sample
preparation and chemical analysis part of their thesis this year.
ASL staff has constructed, and taught individual
instrumentation-oriented classes for four graduate and undergraduate SAES
The educational impacts were the
training of graduate students in instrumental analysis, the serving on
thesis committees and sitting in one oral defense, and the development and
teaching of experiments in teaching labs.
ASL staff analyzed samples for or collaborated with investigators
in ten different ongoing research projects, and one project involving both
extension and research.
The Analytical Services Laboratory
received a significant donation from Lorillard Tobacco Company with an
estimated worth of $250,000. The
donations were for an upgrade of our 270 MHz NMR system components
General Capabilities: Analyses by AOAC, Standard
Methods, EPA, FDA, and customized methodologies for organic, elemental,
and inorganic analysis.
1. UV/VIS Absorption Spectrophotometry
- Ion Chromatography
Equipment: CARY 1E Double Beam UV/VIS
Spectrophotometer with autosampler/sample preparation station. Perkin
Elmer Lambda 3B Double Beam UV/VIS Spectrophotometer.
Lachat Flow Injection Analyzer/ Ion Chromatograph (FIA/IC).
Current Analyses: Most of the analyses are
colorimetric for o-Phosphate, Nitrate/Nitrite, and Ammonium.
Capabilities: Any colorimetric analysis of any
analyte with a derivative absorbing in the region of 200 to 900
nanometers. Any kinetic analysis of an analyte absorbing in the same
region for an ambient-temperature reaction and a reactant half-life on
the order of at least two minutes.
Both anions and cations in potable water (or low ionic strength
aqueous solutions) can be measured with the ion chromatograph.
The technique is similar to HPLC, but at lower liquid pressures.
A. Cary 1E
2. Gas Chromatography and Mass Spectrometry
Equipment: Two Hewlett Packard 5890 (II) gas
chromatographs. One Flame Ionization (FID), two Electron Capture (ECD),
two Nitrogen/Phosphorus (NPD), and one HP5971A mass spectrometer
detectors. TEKMAR 7000 Static Headspace Autosampler, and model
ALS2000/LCS2016 Dynamic Purge-And-Trap controller and autosampler.
Current Analyses: Most analyses are
chromatographic separation and analysis of herbicides, pesticides, their
metabolites, volatile chlorinated hydrocarbons, flavor chemicals in food
analysis, fatty acids.
Capabilities: Volatile and Semivolatile organic
molecule qualitative and quantitative analysis.
(Purge-and-Trap Unit With GC/ECD/FID in Background)
3. Nuclear Magnetic Resonance Spectroscopy
Equipment: JEOL EX-270 MHz NMR with 5 mm and 10 mm
tunable broadband probes and temperature controller for sub and
supra-ambient temperature sample analysis, 5 mm Carbon/Hydrogen probe,
Carbon/Hydrogen solid state probe, Sun O2 workstation.
Recent upgrades (10 mm probe, solid state probe, and Sun
Workstation) were donated by Lorillard Tobacco Co.
Current Analyses: Herbicides and pesticides plus
metabolites, mono and diglycerides, glycoproteins and proteins with
anti-microbial activity. Upcoming
projects are oriented heavily toward glycoproteins analysis and
medicinal Herb/Mushroom active agents.
Capabilities: Compounds with at least
parts-per-million levels (or ability to concentrate sample to this
level) of compounds containing phosphorus, carbon, hydrogen, nitrogen,
aluminum, and chlorine.
D. JEOL EX-270 MHz NMR
Ion Coupled Plasma/Atomic Emission Spectroscopy (ICP/AES)
Elmer Optima 3300 DV with 150 place autosampler.
Phosphorus, Copper, Zinc, Sodium, Calcium, Magnesium, Manganese,
Potassium, Iron, Cadmium, Selenium, Nickel. Upcoming analysis are
elemental analysis of metals in medicinal mushrooms.
Capabilities: The spectrometer is a polychromator
versus a monochromater – meaning that more than one emission
wavelength can be monitored in a parallel fashion. Six thousand emission
wavelength lines that include the major emission lines for 72 of the 110
elements in the periodic chart can be monitored simultaneously. The
inert gases, halogens, and certain rare earth metals cannot be detected
with this instrument.
Perkin Elmer Optima 3300 DV ICP/AES
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